C (3-6 months) Sprouty4 KO, B6-Spry4 KO Sprouty4 KO, B6-Spry4 KO Sprouty4 (Spry4) gene knockout mice. Exon 2 was replaced with a PGK-neo cassette. Homozygous mutant mice exhibit abnormal mandible morphology. Although a significant fraction of homozygotes die shortly after birth due to mandible defects, the remainder are viable and fertile, but postnatal growth is reduced for most homozygous mice. true RBRC05757 B6.Cg-Spry4<tm1Ayos> B6.Cg-Spry4<tm1Ayos> <a href='https://brc.riken.jp/mus/pcr05757'>Genotyping protocol -PCR-</a> Developmental Biology Research Akihiko YOSHIMURA 吉村　昭彦 C（3〜6か月） Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophy.s Res. Commun., 352, 896-902 (2007).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Biochem. Biophy.s Res. Commun., 352, 896-902 (2007).<br>研究成果の公表にあたって謝辞の表明を必要とする。 九州大学生体防御医学研究所・吉村昭彦先生。 Heterozygote x Wild-type [C57BL/6JJcl] Heterozygote x Wild-type [C57BL/6JJcl] Mouse Spry4 genomic DNA, Mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene Developed by Akihiko Yoshimura, Medical Institute of Bioregulation, Kyushu University. Sprouty4 (Spry4) 遺伝子のノックアウトマウス。エクソン2をPGK-neoカセットで置換。ホモマウスは骨格系の異常を呈す。多くは生後致死となる。残りのマウスは生存性/繁殖性あり (成長は不良) 。